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Tredimensionel billeddannelse i høj opløsning af fodpudevaskulaturen i en Murine Hindlimb Gangrene Model
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Journal (Medicine)
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Tredimensionel billeddannelse i høj opløsning af fodpudevaskulaturen i en Murine Hindlimb Gangrene Model

Article DOI: 10.3791/63284-v • 08:16 min • March 16th, 2022
March 16th, 2022 •
1, 1,2, 3, 1, 1,2, 4, 1,2, 1,2, 1,2
1Dewitt Daughtry Family Department of Surgery, University of Miami Miller School of Medicine, 2Vascular Biology Institute, University of Miami Miller School of Medicine, 3University of Miami, 4Analytical Imaging Core Facility, University of Miami

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Den nuværende protokol beskriver en unik, klinisk relevant model af perifer arteriel sygdom, der kombinerer lårbensarterie og veneelektrokoagulering med administration af en nitrogenoxidsyntasehæmmer for at inducere bagbengangren i FVB-mus. Intrakardial DiI-perfusion anvendes derefter til tredimensionel billeddannelse i høj opløsning af fodpudens vaskulatur.

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Medicin udgave 181 Hindlimb iskæmi koldbrand lårbensarterieligation L-NAME DiI perfusion perifer arteriel sygdom neovaskularisering angiogenese
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