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Journal / Biochemistry / 即用型qPCR，用于检测 克氏锥虫…
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Biochemistry

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JoVE Journal Biochemistry
即用型qPCR，用于检测 克氏锥虫 或其他致病微生物的DNA
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Journal (Biochemistry)
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Affinity Chromatography Mediated Fibrinolytic Enzymes Purification from Crude Protein Extracts (Video) | JoVE
Journal (Biochemistry)
Affinity Chromatography Mediated Fibrinolytic Enzymes Purification from Crude Protein Extracts (Video) | JoVE
 
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即用型qPCR，用于检测 克氏锥虫 或其他致病微生物的DNA

Article DOI: 10.3791/63316-v • 05:50 min • January 20th, 2022
January 20th, 2022 •
1,2,3, 1,3, 1,2, 1,3
1Laboratório de Ciências e Tecnologias Aplicadas em Saúde (LaCTAS), Instituto Carlos Chagas (ICC), Fundação Oswaldo Cruz (Fiocruz), 2Pós-graduação em Engenharia de Bioprocessos e Biotecnologia, Universidade Federal do Paraná (UFPR), 3Pós-graduação em Biociências e Biotecnologia, Instituto Carlos Chagas (ICC), Fundação Oswaldo Cruz (Fiocruz)

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本工作描述了生产用于 克氏锥虫 DNA检测的即用型qPCR的步骤，该qPCR可以预先加载到反应容器中并在冰箱中储存数月。

Tags

生物化学，第179期，即用型，PCR，诊断， 锥虫，实验室常规，被忽视的疾病，热带病
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