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Journal / Biochemistry / Kvantifiering av subcellulär glykogenfördelning i …
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Biochemistry

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JoVE Journal Biochemistry
Kvantifiering av subcellulär glykogenfördelning i skelettmuskelfibrer med hjälp av transmissionselektronmikroskopi
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Kvantifiering av subcellulär glykogenfördelning i skelettmuskelfibrer med hjälp av transmissionselektronmikroskopi

Article DOI: 10.3791/63347-v • 08:32 min • February 7th, 2022
February 7th, 2022 •
1, 2, 3, 3, 2
1Research center for applied health science, University College South Denmark, 2Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, 3Department of Biomedical Sciences, Core Facility for Integrated Microscopy, University of Copenhagen

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Ett modifierat efterfixeringsförfarande ökar kontrasten mellan glykogenpartiklar i vävnad. Detta dokument ger ett steg-för-steg-protokoll som beskriver hur man hanterar vävnaden, utför avbildningen och använder stereoologiska metoder för att erhålla opartiska och kvantitativa data om fibertypspecifik subcellulär glykogenfördelning i skelettmuskeln.

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Biokemi utgåva 180
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