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Biochemistry

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JoVE Journal Biochemistry
Kvantifisering av subcellulær glykogenfordeling i skjelettmuskulaturfibre ved hjelp av transmisjonselektronmikroskopi
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Kvantifisering av subcellulær glykogenfordeling i skjelettmuskulaturfibre ved hjelp av transmisjonselektronmikroskopi

Article DOI: 10.3791/63347-v • 08:32 min • February 7th, 2022
February 7th, 2022 •
1, 2, 3, 3, 2
1Research center for applied health science, University College South Denmark, 2Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, 3Department of Biomedical Sciences, Core Facility for Integrated Microscopy, University of Copenhagen

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En modifisert post-fiksering prosedyre øker kontrasten av glykogen partikler i vev. Dette dokumentet gir en trinnvis protokoll som beskriver hvordan man håndterer vevet, utfører avbildningen og bruker stereologiske metoder for å oppnå objektive og kvantitative data om fibertypespesifikk subcellulær glykogenfordeling i skjelettmuskulatur.

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