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Journal / Biochemistry / Kvantificering af subcellulær glykogenfordeling i …
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Biochemistry

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JoVE Journal Biochemistry
Kvantificering af subcellulær glykogenfordeling i skeletmuskelfibre ved hjælp af transmissionselektronmikroskopi
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Kvantificering af subcellulær glykogenfordeling i skeletmuskelfibre ved hjælp af transmissionselektronmikroskopi

Article DOI: 10.3791/63347-v • 08:32 min • February 7th, 2022
February 7th, 2022 •
1, 2, 3, 3, 2
1Research center for applied health science, University College South Denmark, 2Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, 3Department of Biomedical Sciences, Core Facility for Integrated Microscopy, University of Copenhagen

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En modificeret postfikseringsprocedure øger kontrasten mellem glykogenpartikler i væv. Dette papir giver en trinvis protokol, der beskriver, hvordan man håndterer vævet, udfører billeddannelsen og bruger stereologiske metoder til at opnå upartiske og kvantitative data om fibertypespecifik subcellulær glykogenfordeling i skeletmuskulaturen.

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Biokemi udgave 180
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