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Journal / Biochemistry / Övervakning av proteinaggregeringskinetik in vivo …
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Biochemistry

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JoVE Journal Biochemistry
Övervakning av proteinaggregeringskinetik in vivo med hjälp av automatiserad inkluderingsräkning i Caenorhabditis elegans
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Övervakning av proteinaggregeringskinetik in vivo med hjälp av automatiserad inkluderingsräkning i Caenorhabditis elegans

Article DOI: 10.3791/63365-v • 06:49 min • December 17th, 2021
December 17th, 2021 •
*1, *1, *1, 1
1Bijvoet Centre for Biomolecular Research, Utrecht University
* These authors contributed equally

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Här presenteras en metod för analys av proteinaggregeringskinetik i nematoden Caenorhabditis elegans. Djur från en ålderssynkronerad population avbildas vid olika tidpunkter, följt av halvautomatisk inkluderingsräkning i CellProfiler och anpassning till en matematisk modell i AmyloFit.

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Biokemi utgåva 178
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