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Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
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Biochemistry
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JoVE Journal Biochemistry
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

DOI:
10.3791/55218-v

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10:05 min

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January 16, 2017

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, ,
1University Children’s Hospital Basel (UKBB),University of Basel, 2Department of Clinical Research,University of Basel

Chapters

  • 00:05Title
  • 01:01Co-immunoprecipitation
  • 04:14Immunocytochemistry
  • 06:15Proximity Ligation Assay
  • 08:19Results: Visualization of NF90-RBM3 Interactions in Nuclear and Cytoplasmic Fractions of Cells
  • 09:14Conclusion

Summary

Automatic Translation

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Protein-protein interactions can occur in both the nucleus and the cytoplasm of a cell. To investigate these interactions, traditional co-immunoprecipitation and modern proximity ligation assay are applied. In this study, we compare these two methods to visualize the distribution of NF90-RBM3 interactions in the nucleus and the cytoplasm.

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Keywords: Protein-protein InteractionCo-immunoprecipitationIn Situ Proximity Ligation AssayNuclear FractionCytoplasmic FractionCellular CompartmentsHEK293 CellsCytoplasmic ExtractionNuclear ExtractionProtein G Magnetic BeadsInput Samples

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