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JoVE Journal
Biochemistry

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Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
 

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Article DOI: 10.3791/57479
April 20th, 2018

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Summary April 20th, 2018

We provide a step-by-step protocol for split-BioID, a protein fragments-complementation assay based on the proximity-labeling technique BioID. Activated on the interaction of two given proteins, it allows the proteomics analysis of context-dependent protein complexes in their native cellular environment. The method is simple, cost-effective and only requires standard laboratory equipment.

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