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Optisk rydning af plantevæv til fluorescensbilleddannelse
JoVE Journal
Biology
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JoVE Journal Biology
Optical Clearing of Plant Tissues for Fluorescence Imaging

Optisk rydning af plantevæv til fluorescensbilleddannelse

DOI:
10.3791/63428-v

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04:55 min

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January 05, 2022

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, , , ,
1Institute of Transformative Bio-Molecules (ITbM),Nagoya University, 2Institute for Advanced Research (IAR),Nagoya University, 3Division of Biological Science, Graduate School of Science,Nagoya University, 4Department of Biological Sciences, Graduate School of Science,The University of Tokyo

Chapters

  • 00:05Introduction
  • 00:48Fixation of Samples
  • 01:48Clearing
  • 02:21Observation
  • 02:59Results: Optical Clearing and Fluorescence Imaging of Tissues
  • 04:18Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskrives en metode til at gøre plantevæv gennemsigtigt og samtidig opretholde stabiliteten af fluorescerende proteiner. Denne teknik letter dyb billeddannelse af ryddet plantevæv uden fysisk sektionering.

Tags

Optical ClearingFluorescence ImagingWhole-plant ImagingPlant-microbe InteractionsFixationFluorescent ProteinVacuum InfiltrationClearing SolutionClearSeePBSSilicone FrameFluorescent Microscopy

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