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JoVE Journal Bioengineering
Un approccio non sequenziale per il rilevamento rapido dell'editing dell'RNA
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Un approccio non sequenziale per il rilevamento rapido dell'editing dell'RNA

Article DOI: 10.3791/63591-v • 08:50 min • April 21st, 2022
April 21st, 2022 •
1, 1, 1,2
1Department of Bioscience and Biotechnology, Japan Advanced Institute of Science and Technology, 2BioSeeds Corporation, JAIST Venture Business Laboratory, Ishikawa Create Labo

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Il rilevamento rapido e la quantificazione affidabile degli eventi di modifica dell'RNA su scala genomica rimangono impegnativi e attualmente si basano su metodi di sequenziamento diretto dell'RNA. Il protocollo qui descritto utilizza l'elettroforesi su gel a gradiente di microtemperatura (μTGGE) come metodo semplice, rapido e portatile per rilevare l'editing dell'RNA.

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Bioingegneria Numero 182
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