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Journal / Biochemistry / Rensing av allestedsnærværende p53-proteiner fra…
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Biochemistry

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JoVE Journal Biochemistry
Rensing av allestedsnærværende p53-proteiner fra pattedyrceller
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Rensing av allestedsnærværende p53-proteiner fra pattedyrceller

Article DOI: 10.3791/63602-v • 10:55 min • March 21st, 2022
March 21st, 2022 •
1, 1, 1
1Center for Translational Medicine, The Affiliated Zhangjiagang Hospital of Soochow University

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Protokollen beskriver en trinnvis metode for å rense ubiquitinerte proteiner fra pattedyrceller ved hjelp av p53 tumor suppressor protein som et eksempel. Ubiquitinated p53 proteiner ble renset fra celler under strenge nondenaturing og denaturing forhold.

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