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Konstruktion homozygoter Mutanten der Wanderheuschrecke mittels CRISPR/Cas9-Technologie
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Konstruktion homozygoter Mutanten der Wanderheuschrecke mittels CRISPR/Cas9-Technologie

Article DOI: 10.3791/63629-v • 10:07 min • March 16th, 2022
March 16th, 2022 •
*1, *2,3, 2,3, 1, 2,3, 2,3, 2, 2
1State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 2Research Institute of Applied Biology, Shanxi University, 3School of Life Science, Shanxi University
* These authors contributed equally

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Diese Arbeit liefert ein systematisch optimiertes Verfahren zur CRISPR/Cas9-Ribonuklease-basierten Konstruktion homozygoter Heuschreckenmutanten sowie eine detaillierte Methode zur Kryokonservierung und Reanimation der Heuschreckeneier.

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Retraktion Ausgabe 181 homozygote Mutanten wandernde Heuschrecken CRISPR/Cas9 Genomeditierung Mikroinjektion Kryokonservierung und Wiederbelebung von Eizellen
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