Journal
/
Biochemistry
/
Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

DOI:
10.3791/60815-v

•

08:22 min

•

January 22, 2020

•

, ,
1Department of Pharmacology,Weill Cornell Medical College

Chapters

  • 00:04Title
  • 00:32Sensor Cartridge and Extracellular Flux Analyzer Calibrant, Concanavalin A (ConA)-Coated Microplate, and Sperm Buffer Preparation
  • 01:52Cartridge Calibration Preparation and Wave Template Generation
  • 03:00Mouse Sperm Isolation
  • 03:42Sensor Cartridge Loading
  • 04:59Sperm Plate Preparation
  • 06:17Results: Representative Changes in Glycolysis and Oxidative Phosphorylation During Mouse Sperm Capacitation
  • 07:42Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the application of an extracellular flux analyzer to monitor real-time changes in glycolysis and oxidative phosphorylation during mouse sperm capacitation.

Tags

Keywords: Extracellular Flux AnalyzerGlycolysisOxidative PhosphorylationMouse SpermCapacitationConATYH BufferWave TemplateSperm Metabolism

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/60815?article_type=v

Oligopeptide Competition Assay for Phosphorylation Site Determination
check_url/60815?article_type=v

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
check_url/60815?article_type=v

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes
check_url/60815?article_type=v

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
check_url/60815?article_type=v

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson’s Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation
check_url/60815?article_type=v

Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress
check_url/60815?article_type=v

Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
check_url/60815?article_type=v

DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density
check_url/60815?article_type=v

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
check_url/60815?article_type=v

Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo

Read Article