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Dissekere, fikse og visualisere Drosophila Pupal Notum
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Biology
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JoVE Journal Biology
Dissecting, Fixing, and Visualizing the Drosophila Pupal Notum

Dissekere, fikse og visualisere Drosophila Pupal Notum

DOI:
10.3791/63682-v

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09:07 min

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April 06, 2022

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, ,
1Dept. Cell and Developmental Biology,Vanderbilt University, 2Program in Developmental Biology,Vanderbilt University

Chapters

  • 00:04Introduction
  • 00:54Pupae Immobilization
  • 03:07Dissecting the Notum
  • 05:59Mounting the Notum
  • 07:47Results: Imaging of Notum
  • 08:27Conclusion

Summary

Automatic Translation

Automatic Translation

Den nåværende protokollen beskriver forberedelse og visualisering av fast vev av Drosophila pupal notum. Den kan brukes til enten intakt eller såret vev, og vevets opprinnelige arkitektur er bevart. Prosedyrene for dissekering, fiksering og farging er alle beskrevet i denne artikkelen.

Tags

Keywords: DrosophilaPupaNotumImmunohistochemistryDissectionLive-imagingAntibody StainingDNA StainingIn Situ HybridizationMicroscopyForcepsAdhesive GluePBSPipette

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