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JoVE Journal Neuroscience
Celletypespecifik proteinrensning og identifikation fra komplekse væv ved hjælp af en mutant methionin tRNA-syntetasemuselinje
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Celletypespecifik proteinrensning og identifikation fra komplekse væv ved hjælp af en mutant methionin tRNA-syntetasemuselinje

Article DOI: 10.3791/63713-v • 07:39 min • April 13th, 2022
April 13th, 2022 •
1, 2, 2, 1, 1, 1, 2
1Max-Planck-Institute for Brain Research, Synaptic Plasticity Department, 2Department of Biochemistry and Molecular Biology, Veterinary School, Complutense University of Madrid

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Denne protokol beskriver, hvordan man udfører celletypespecifik proteinmærkning med azidonorleucin (ANL) ved hjælp af en muselinje, der udtrykker en mutant L274G-methionin tRNA-syntetase (MetRS *) og de nødvendige trin til mærkning af celletypespecifikke proteiner. Vi skitserer to mulige ANL-administrationsveje i levende mus ved (1) drikkevand og (2) intraperitoneale injektioner.

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Neurovidenskab udgave 182
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