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Journal / Neuroscience / 使用突变蛋氨酸tRNA合成酶小鼠系从复杂组织中纯化和鉴定细胞类型特异性蛋白…
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Neuroscience

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JoVE Journal Neuroscience
使用突变蛋氨酸tRNA合成酶小鼠系从复杂组织中纯化和鉴定细胞类型特异性蛋白
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使用突变蛋氨酸tRNA合成酶小鼠系从复杂组织中纯化和鉴定细胞类型特异性蛋白

Article DOI: 10.3791/63713-v • 07:39 min • April 13th, 2022
April 13th, 2022 •
1, 2, 2, 1, 1, 1, 2
1Max-Planck-Institute for Brain Research, Synaptic Plasticity Department, 2Department of Biochemistry and Molecular Biology, Veterinary School, Complutense University of Madrid

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该协议描述了如何使用表达突变L274G-蛋氨酸tRNA合成酶（MetRS *）的小鼠系使用叠氮金亮氨酸（ANL）进行细胞类型特异性蛋白质标记，以及标记细胞类型特异性蛋白质分离的必要步骤。我们通过（1）饮用水和（2）腹膜内注射概述了活小鼠中两种可能的ANL给药途径。

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神经科学，第182期，
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