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Journal / Medicine / Durchflusszytometrische Analyse von Biomarkern…
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Durchflusszytometrische Analyse von Biomarkern zum Nachweis von Funktionsdefekten menschlicher Spermien
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Durchflusszytometrische Analyse von Biomarkern zum Nachweis von Funktionsdefekten menschlicher Spermien

Article DOI: 10.3791/63790-v • 08:48 min • April 21st, 2022
April 21st, 2022 •
1, 1, 1, 1, 2, 1, 1, 1, 1, 1
1Institute of Toxicology, College of Preventive Medicine, Army Medical University (Third Military Medical University), 2Department of Chemical Defense Medicine, College of Military Preventive Medicine, Army Medical University (Third Military Medical University)

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Das vorliegende Protokoll bietet eine praktische Lösung, die die Messung von Apoptose, mitochondrialem Membranpotenzial und DNA-Schäden in menschlichen Spermien mit einem einzigen Zytometer ermöglicht.

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Durchflusszytometrie Biomarker Funktionsdefekte der Spermien konventionelle Samenparameter idiopathische Unfruchtbarkeit Spermienapoptose Mitochondrialmembranpotenzial (MMP) DNA-Schäden FCM-Techniken Annexin-V-Fluorescein-Isothiocyanat (FITC)/Propidiumiodid (PI)-Färbung 5,5& 8242;,6,6&8242;-Tetrachlor-1,1&8242;,3,3&8242;-Tetraethyl-benzimidazolylcarbocyanin-Iodid-Sonde (JC-1) Spermienchromatin-Struktur-Assay (SCSA) Acridinorange (AO)-Färbung
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