JoVE Journal
Developmental Biology
Developmental Biology
A subscription to JoVE is required to view this content.
Chapters
Summary
This versatile protocol describes the isolation of premigratory neural crest cells (NCCs) through the excision of cranial neural folds from chick embryos. Upon plating and incubation, migratory NCCs emerge from neural fold explants, allowing for assessment of cell morphology and migration in a simplified 2D environment.