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Preparação de Proteômica de Célula Única para Análise de Espectrometria de Massa usando Lise de Calor Congelante e um Transportador Isobárico
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Preparação de Proteômica de Célula Única para Análise de Espectrometria de Massa usando Lise de Calor Congelante e um Transportador Isobárico

Article DOI: 10.3791/63802-v • 06:13 min • December 9th, 2022
December 9th, 2022 •
*1, 1,2,3, *1
1Department of Bioengineering, Northeastern University, 2Barnett Institute, Northeastern University, 3Department of Biology, Northeastern University
* These authors contributed equally

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Neste protocolo, descrevemos como preparar células de mamíferos para análise proteômica unicelular via espectrometria de massa usando reagentes e equipamentos comercialmente disponíveis, com opções para pipetagem manual e automática.

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Biologia Edição 190
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