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Preparación de proteómica unicelular para análisis de espectrometría de masas utilizando lisis por congelación y calor y un portador isobárico
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Preparación de proteómica unicelular para análisis de espectrometría de masas utilizando lisis por congelación y calor y un portador isobárico

Article DOI: 10.3791/63802-v • 06:13 min • December 9th, 2022
December 9th, 2022 •
*1, 1,2,3, *1
1Department of Bioengineering, Northeastern University, 2Barnett Institute, Northeastern University, 3Department of Biology, Northeastern University
* These authors contributed equally

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En este protocolo, describimos cómo preparar células de mamíferos para el análisis proteómico unicelular mediante espectrometría de masas utilizando reactivos y equipos disponibles comercialmente, con opciones para pipeteo manual y automático.

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Biología Número 190
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