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Journal / Neuroscience / Mezoskopikten Mikroskobik Ölçeklere Nöronal…
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Neuroscience

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JoVE Journal Neuroscience
Mezoskopikten Mikroskobik Ölçeklere Nöronal Görüntüleme için Bir Doku Temizleme Yöntemi
A Visual Guide to Sorting Electrophysiological Recordings Using 'SpikeSorter'
Journal (Neuroscience)
A Visual Guide to Sorting Electrophysiological Recordings Using 'SpikeSorter'
Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits
Journal (Neuroscience)
Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits
 
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Mezoskopikten Mikroskobik Ölçeklere Nöronal Görüntüleme için Bir Doku Temizleme Yöntemi

Article DOI: 10.3791/63941-v • 07:20 min • May 10th, 2022
May 10th, 2022 •
1,2, 1,2,3, 1,2,4,5, 2,3, 6, 1,2,7
1Department of Neuroanatomy, Juntendo University Graduate School of Medicine, 2Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, 3Advanced Research Institute for Health Sciences, Juntendo University, 4Department of Neuroscience, Graduate School of Medicine, Kyoto University, 5Japan Society for the Promotion of Science, 6Department of Oral Anatomy and Neurobiology, Graduate School of Dentistry, Osaka University, 7Department of Multi-Scale Brain Structure Imaging, Juntendo University Graduate School of Medicine

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Protokol, bir doku temizleme yöntemi olan ScaleSF kullanılarak beyin diliminde ayrıntılı bir nöronal görüntüleme yöntemi sağlar. Protokol, beyin dokusu hazırlama, doku berraklaştırma, temizlenmiş dilimlerin işlenmesi ve nöronal yapıların mezoskopikten mikroskobik seviyelere kadar konfokal lazer taramalı mikroskopi görüntülemesini içerir.

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