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Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography
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Biochemistry
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JoVE Journal Biochemistry
Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

DOI:
10.3791/62201-v

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08:30 min

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February 02, 2021

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,
1Department of Cell Biology,University of Texas Southwestern Medical Center

Chapters

  • 00:04Introduction
  • 00:30Growth and Labelling of Yeast Cells
  • 02:55Isolation of Total Lipids from Yeast
  • 04:42Thin Layer Chromatography and Quantification of Results
  • 07:09Results: 14C-Acetic Acid Radiolabeling Detection Analysis of Multiple NL Species
  • 08:03Conclusion

Summary

Automatic Translation

Automatic Translation

Here, a protocol is presented for the metabolic labeling of yeast with 14C-acetic acid, which is coupled with thin layer chromatography for the separation of neutral lipids.

Tags

Keywords: Metabolic LabelingThin Layer ChromatographyLipid MetabolismSaccharomyces CerevisiaeYeast CultureRadiolabelingQuenching BufferCentrifugationLipid SynthesisEnzyme Regulation

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