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Biochemistry

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JoVE Journal Biochemistry
Semi-automatisert fenotypisk analyse av funksjonelle 3D sfæroidcellekulturer
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Semi-automatisert fenotypisk analyse av funksjonelle 3D sfæroidcellekulturer

Article DOI: 10.3791/65086-v • 06:48 min • August 18th, 2023
August 18th, 2023 •
1, 1, 2,3, 2, 3, 1
1Department of Biochemistry, Albert Einstein College of Medicine, 2Department of Biochemistry and Molecular Biology, University of Southern Denmark, 3CelVivo ApS

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Vi presenterer en protokoll for dyrking av høyreproduserbare sfæroider og deres fenotypiske karakterisering ved hjelp av bildeopptak og proteomikk.

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Biokjemi utgave 198 Clinostat Inkubator 3D cellekulturer kreft Hepatocytter ikke-kreft hepatocytter leverceller inkubatorer for 3D-celler Cell Growth Monitoring Cell Growth Snapshots Spheroid Counting Spheroid Measurement THLE-3 Cell Line HepG2/C3A Cell Line Proteomics Experiments Cell Signaling Perturbation Respiratory Chain Metabolism Metal Detoxification Fenotypisk karakterisering Image Capture Proteomics Pipeline
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