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Journal
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Genetics
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测量
秀丽隐杆线虫
对乙酰胆碱受体激动剂左旋咪唑的敏感性
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
Measuring
Caenorhabditis elegans
Sensitivity to the Acetylcholine Receptor Agonist Levamisole
Please note that all translations are automatically generated.
Click here for the English version.
测量
秀丽隐杆线虫
对乙酰胆碱受体激动剂左旋咪唑的敏感性
DOI:
10.3791/64056-v
•
08:11 min
•
June 07, 2022
•
Allison N. Davis
,
Jessica E. Tanis
1
Department of Biological Sciences
,
University of Delaware
Chapters
00:05
Introduction
00:45
Preparation of Plates for the Levamisole Assay
02:03
Synchronizing
C. elegans
03:42
Plating Synchronized
C. elegans
04:36
Performing the Levamisole Assay
05:47
Data Analysis
06:52
Results: Levamisole-Induced Paralysis in Wild Type and Representative Mutant
C. elegans
07:28
Conclusion
Summary
Automatic Translation
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Automatic Translation
本方案描述了一种测定以确定对左旋咪唑的反应,左旋咪唑是一类
秀丽隐杆线虫乙酰
胆碱受体的药理激动剂。在这种液体左旋咪唑游泳测定中,研究人员目视观察和定量在24孔板中培养的动物的时间依赖性麻痹。
Tags
Caenorhabditis Elegans
Levamisole
Acetylcholine Receptor Agonist
Neuromuscular Junction
Paralysis
Nematode Growth Media
OP50
Synchronization
Bleaching Solution
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