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Journal / Bioengineering / Captação e Descelularização de Membros…
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Bioengineering

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JoVE Journal Bioengineering
Captação e Descelularização de Membros Posteriores de Rato Usando Biorreator Ex Vivo à Base de Perfusão para Alotransplante Composto Vascularizado
Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
Journal (Bioengineering)
Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
Sandwich-like Microenvironments to Harness Cell/Material Interactions
Journal (Bioengineering)
Sandwich-like Microenvironments to Harness Cell/Material Interactions
 
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Captação e Descelularização de Membros Posteriores de Rato Usando Biorreator Ex Vivo à Base de Perfusão para Alotransplante Composto Vascularizado

Article DOI: 10.3791/64069-v • 08:50 min • June 9th, 2022
June 9th, 2022 •
1,2, 1,3, 1,2,4
1Latner Thoracic Research Surgical Laboratories, University Health Network, Toronto General Hospital, 2Institute of Medical Science, Temerty Faculty of Medicine, University of Toronto, 3Institute of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 4Division of Plastic & Reconstructive Surgery, Department of Surgery, University of Toronto

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Descrevemos a técnica cirúrgica e o processo de descelularização de membros posteriores compostos de ratos. A descelularização é realizada usando dodecil sulfato de sódio de baixa concentração através de um sistema de perfusão de máquina ex vivo.

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Bioengenharia Edição 184
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