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JoVE Journal Bioengineering
Upphandling och decellularisering av råtta bakben med hjälp av en ex vivo perfusionsbaserad bioreaktor för vaskulariserad kompositallotransplantation
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Upphandling och decellularisering av råtta bakben med hjälp av en ex vivo perfusionsbaserad bioreaktor för vaskulariserad kompositallotransplantation

Article DOI: 10.3791/64069-v • 08:50 min • June 9th, 2022
June 9th, 2022 •
1,2, 1,3, 1,2,4
1Latner Thoracic Research Surgical Laboratories, University Health Network, Toronto General Hospital, 2Institute of Medical Science, Temerty Faculty of Medicine, University of Toronto, 3Institute of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 4Division of Plastic & Reconstructive Surgery, Department of Surgery, University of Toronto

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Vi beskriver den kirurgiska tekniken och decellulariseringsprocessen för sammansatta råtta bakben. Decellularisering utförs med användning av natriumdodecylsulfat med låg koncentration genom ett ex vivo-maskinperfusionssystem.

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Bioengineering utgåva 184
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