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Aislamiento, caracterización y aplicaciónerapéutica de vesículas extracelulares a partir de células madre mesenquimales humanas cultivadas…
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Aislamiento, caracterización y aplicación terapéutica de vesículas extracelulares a partir de células madre mesenquimales humanas cultivadas
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Article
DOI:
10.3791/64135-v
•
07:03 min
•
September 23rd, 2022
Aislamiento, caracterización y aplicación terapéutica de vesículas extracelulares a partir de células madre mesenquimales humanas cultivadas
September 23rd, 2022
•
Shu-Juan Xing1,2, Kai-Chao Zhang2,3, Si-Yuan Tang2,4, Lu Liu2,5, Yuan Cao2,5, Chen-Xi Zheng2, Bing-Dong Sui2, Yan Jin2,3
1College of Life Science, Northwest University, 2State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, 3Xi'an Institute of Tissue Engineering and Regenerative Medicine, 4School of Basic Medicine, Fourth Military Medical University, 5Department of Orthodontics, School of Stomatology, Fourth Military Medical University
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Summary
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El presente protocolo describe la centrifugación diferencial para aislar y caracterizar EVs representativas (exosomas y microvesículas) de MSCs humanas cultivadas. Otras aplicaciones de estos vehículos eléctricos también se explican en este artículo.