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Isolamento de células endoteliais glomerulares de camundongos imortalizadas condicionalmente com mitocôndrias fluorescentes
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Isolamento de células endoteliais glomerulares de camundongos imortalizadas condicionalmente com mitocôndrias fluorescentes

Article DOI: 10.3791/64147-v • 11:49 min • September 13th, 2022
September 13th, 2022 •
1, 1, 1, 1
1Department of Medicine, Division of Nephrology, The Icahn School of Medicine at Mount Sinai

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O artigo descreve o método de isolamento de células endoteliais glomerulares imortalizadas condicionalmente dos rins de camundongos transgênicos expressando o vírus símio termolábil 40 e mitocôndrias fotoativáveis,excisadas por PhAM. Descrevemos o procedimento para isolamento de glomérulos de rins inteiros usando contas, etapas de digestão, semeadura e cultura de GECs-CD31 positivos.

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Biologia Edição 187
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