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Journal / Neuroscience / In vivo Imágenes de calcio de campo amplio y dos…
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Neuroscience

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JoVE Journal Neuroscience
In vivo Imágenes de calcio de campo amplio y dos fotones de un ratón usando una ventana craneal grande
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In vivo Imágenes de calcio de campo amplio y dos fotones de un ratón usando una ventana craneal grande

Article DOI: 10.3791/64224-v • 06:45 min • August 4th, 2022
August 4th, 2022 •
1, 2,3, 4, 2,3, 1
1Department of Neurophysiology, Faculty of Medicine, University of Yamanashi, 2Department of Neuropharmacology, Faculty of Medicine, University of Yamanashi, 3Yamanashi GLIA center, Interdisciplinary Graduate School of Medicine, University of Yamanashi, 4Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo

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El presente protocolo describe la fabricación de una ventana craneal grande (6 x 3 mm2) utilizando envoltura de alimentos, silicona transparente y vidrio de cubierta. Esta ventana craneal permite experimentos in vivo de imágenes de calcio de campo amplio y dos fotones en el mismo ratón.

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Neurociencia Número 186
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