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06:45 min
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August 04, 2022
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Summary
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本プロトコルは、フードラップ、透明シリコーン、およびカバーガラスを使用して、大きな(6 x 3 mm2)頭蓋窓を作成することについて説明しています。この頭蓋窓は、同じマウスでの in vivo 広視野および2光子カルシウムイメージング実験を可能にします。
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Cite this Article
Manita, S., Shigetomi, E., Bito, H., Koizumi, S., Kitamura, K. In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window. J. Vis. Exp. (186), e64224, doi:10.3791/64224 (2022).
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