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Rask kolonidannende enhetstelling i 96-brønns plateformat påført Drosophila-mikrobiomet
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Rask kolonidannende enhetstelling i 96-brønns plateformat påført Drosophila-mikrobiomet

Article DOI: 10.3791/64298-v • 12:55 min • January 13th, 2023
January 13th, 2023 •
1, 1,2
1Department of Embryology, Carnegie Institution for Science, 2Department of Biology, Johns Hopkins University

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Denne metoden kvantifiserer mikrobiell overflod ved hjelp av et 96-brønns plateformat til platekolonidannende enheter (CFUer) og påføres Drosophila-mikrobiomet i hele fluehomogenatprøver. CFU-er telles med en automatisert bildeanalyseprogramvare som tilbys her.

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