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Hurtig kolonidannende enhedstælling i 96-brønds pladeformat anvendt på Drosophila-mikrobiomet
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Biology
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JoVE Journal Biology
Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome

Hurtig kolonidannende enhedstælling i 96-brønds pladeformat anvendt på Drosophila-mikrobiomet

DOI:
10.3791/64298-v

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12:55 min

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January 13, 2023

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,
1Department of Embryology,Carnegie Institution for Science, 2Department of Biology,Johns Hopkins University

Chapters

  • 00:04Introduction
  • 01:28Homogenizing Flies Individually in a 96-Well PCR Plate
  • 04:13Serial Dilution of Fly Homogenate and Spotting onto CFU Plates
  • 06:03Quantification of CFUs
  • 06:44Automated Colony Counting Using the Count-On-It Suite
  • 09:57Results: Enumeration of CFUs Using Automated Colony Counting
  • 12:10Conclusion

Summary

Automatic Translation

Automatic Translation

Denne metode kvantificerer mikrobiel overflod ved hjælp af et 96-brønds pladeformat til pladekolonidannende enheder (CFU'er) og anvendes på Drosophila-mikrobiomet i hele fluehomogenatprøver. CFU'er tælles med en automatiseret billedanalysesoftware, der leveres her.

Tags

Colony Forming UnitCFUMicrobiomeDrosophilaHigh-throughputAutomated CountingGnotobiotic96-well PlateMicrofluidicsMicrobiologyMicrobial Quantification

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