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Journal
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Biology
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应用于
果蝇
微生物组的 96 孔板形式的快速菌落形成单位计数
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the
Drosophila
Microbiome
Please note that all translations are automatically generated.
Click here for the English version.
应用于
果蝇
微生物组的 96 孔板形式的快速菌落形成单位计数
DOI:
10.3791/64298-v
•
12:55 min
•
January 13, 2023
•
Ren Dodge
,
William B. Ludington
2
1
Department of Embryology
,
Carnegie Institution for Science
,
2
Department of Biology
,
Johns Hopkins University
Chapters
00:04
Introduction
01:28
Homogenizing Flies Individually in a 96-Well PCR Plate
04:13
Serial Dilution of Fly Homogenate and Spotting onto CFU Plates
06:03
Quantification of CFUs
06:44
Automated Colony Counting Using the Count-On-It Suite
09:57
Results: Enumeration of CFUs Using Automated Colony Counting
12:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方法使用96孔板形式量化微生物丰度,以板菌落形成单位(CFU),并应用于整个果蝇匀浆样品中的
果蝇
微生物组。CFU使用此处提供的自动图像分析软件进行计数。
Tags
Colony Forming Unit
CFU
Microbiome
Drosophila
High-throughput
Automated Counting
Gnotobiotic
96-well Plate
Microfluidics
Microbiology
Microbial Quantification
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