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Journal / Developmental Biology / Effektiv genomredigering af mus ved…
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JoVE Journal Developmental Biology
Effektiv genomredigering af mus ved CRISPR-elektroporation af zygoter
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Journal (Developmental Biology)
In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae
Live-imaging of the Drosophila Pupal Eye
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Effektiv genomredigering af mus ved CRISPR-elektroporation af zygoter

Article DOI: 10.3791/64302-v • 07:17 min • December 16th, 2022
December 16th, 2022 •
1, 1
1Department of Biomedical Sciences, School of Veterinary Medicine, The University of Pennsylvania

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Her beskriver vi en simpel teknik beregnet til effektiv generering af genetisk modificerede mus kaldet CRISPR RNP Electroporation of Zygotes (CRISPR-EZ). Denne metode leverer redigering af reagenser ved elektroporation i embryoner med en effektivitet, der nærmer sig 100%. Denne protokol er effektiv til punktmutationer, små genomiske indsættelser og deletioner i pattedyrembryoner.

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Udviklingsbiologi nummer 190
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