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Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

DOI:
10.3791/64365-v

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09:23 min

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March 10, 2023

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, , , , , , , , , ,
1Utrecht Regenerative Medicine Center, Circulatory Health Laboratory, University Utrecht, Department of Cardiology,University Medical Center Utrecht, 2Amsterdam Cardiovascular Sciences, Department of Physiology,Amsterdam University Medical Center

Chapters

  • 00:04Introduction
  • 00:28Cardiac Spheroid Generation
  • 02:40Cryopreservation of Cardiac Spheroids (CSs)
  • 03:58Thawing of Cardiac Spheroids
  • 04:58Assessment of Intracellular Ca2+ Transients
  • 06:18Results: Generation and Cryopreservation of Cardiac Spheroids from hiPSC‐CMs
  • 08:34Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a set of protocols for the generation and cryopreservation of cardiac spheroids (CSs) from human-induced pluripotent stem cell-derived cardiomyocytes cultured in a high-throughput, multidimensional format. This three-dimensional model functions as a robust platform for disease modeling, high-throughput screenings, and maintains its functionality after cryopreservation.

Tags

Keywords: Cardiac SpheroidsHuman-induced Pluripotent Stem CellsCardiomyocytesHigh-throughput ScreeningBiobankingCell CultureCell DissociationCell CountingSpheroid FormationSpheroid MaintenanceCryopreservation

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