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JoVE Journal Biochemistry
Bepaling van in vitro en cellulaire inschakelkinetiek voor fluorogene RNA-aptameren
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Bepaling van in vitro en cellulaire inschakelkinetiek voor fluorogene RNA-aptameren

Article DOI: 10.3791/64367-v • 08:11 min • August 9th, 2022
August 9th, 2022 •
*1, *1, 1
1Department of Chemistry and Henry Eyring Center for Cell & Genome Science, University of Utah
* These authors contributed equally

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Het protocol presenteert twee methoden om de kinetiek van de fluorogene RNA-aptameren Spinazie2 en Broccoli te bepalen. De eerste methode beschrijft hoe fluorogene aptamerkinetiek in vitro kan worden gemeten met een platenlezer, terwijl de tweede methode de meting van fluorogene aptamerkinetiek in cellen door flowcytometrie beschrijft.

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Biochemie Nummer 186 Fluorescentie flowcytometrie platenlezer Spinazie2 aptamer Broccoli aptamer E. coli
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