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Biochemistry

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JoVE Journal Biochemistry
Bestemmelse af in vitro - og cellulær tændingskinetik til fluorogene RNA-aptamerer
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Bestemmelse af in vitro - og cellulær tændingskinetik til fluorogene RNA-aptamerer

Article DOI: 10.3791/64367-v • 08:11 min • August 9th, 2022
August 9th, 2022 •
*1, *1, 1
1Department of Chemistry and Henry Eyring Center for Cell & Genome Science, University of Utah
* These authors contributed equally

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Protokollen præsenterer to metoder til bestemmelse af kinetikken af de fluorogene RNA-aptamerer Spinat2 og Broccoli. Den første metode beskriver, hvordan man måler fluorogen aptamerkinetik in vitro med en pladelæser, mens den anden metode beskriver målingen af fluorogen aptamerkinetik i celler ved flowcytometri.

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Biokemi udgave 186 fluorescens flowcytometri pladelæser spinat2 aptamer broccoliaptamer E. coli
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