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荧光激活神经元核负分选结合单核RNA测序研究海马神经源性生态位
 
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荧光激活神经元核负分选结合单核RNA测序研究海马神经源性生态位

Article DOI: 10.3791/64369-v 08:16 min October 20th, 2022
October 20th, 2022

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Summary

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这里介绍的是从小鼠齿状回分离的单个细胞核测序的方法,该方法通过荧光激活细胞核(FAN)分选排除大多数神经元。这种方法可生成高质量的表达谱,并促进对生态位中代表的大多数其他细胞类型的研究,包括神经干细胞等稀缺群体。

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神经科学,第188期,
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