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AirID-baserad närhetsmärkning för protein-proteininteraktion i växter
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AirID-baserad närhetsmärkning för protein-proteininteraktion i växter

Article DOI: 10.3791/64428-v • 08:36 min • September 16th, 2022
September 16th, 2022 •
*1, *1, 1, 1, 1
1College of Bioscience and Biotechnology, Yangzhou University
* These authors contributed equally

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Här presenterar vi ett steg-för-steg-protokoll för att utföra PL-experimentet (Proximity Labeling) i gurka (Cucumis sativus L.) med AT4G18020 (APRR2)-AirID-protein som modell. Metoden beskriver konstruktionen av en vektor, omvandlingen av en konstruktion genom agroinfiltration, biotininfiltration, proteinextraktion och rening av biotinmärkta proteiner genom affinitetsreningsteknik.

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AirID närhetsmärkning protein-proteininteraktion PL-metoder modifierat askorbatperoxidas APEX Escherichia coli Biotinligas BirA BioID TurboID restriktioner ny version växtsystem AT4G18020 (APRR2) Protein steg-för-steg-protokoll vektorkonstruktion agroinfiltration biotintransformation proteinextraktion affinitetsreningsteknik analys av protonpumpshämmare i växter
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