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Journal / Cancer Research / Multiparametrisk tumororganoid…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Multiparametrisk tumororganoid lægemiddelscreening ved hjælp af widefield levende cellebilleddannelse til bulk- og enkeltorganoidanalyse
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Multiparametrisk tumororganoid lægemiddelscreening ved hjælp af widefield levende cellebilleddannelse til bulk- og enkeltorganoidanalyse

Article DOI: 10.3791/64434-v • 12:41 min • December 23rd, 2022
December 23rd, 2022 •
1, 2, 1, 1, 1, 1,3, 2, 1,4, 1,4, 1,5, 1
1Center for Oncological Research (CORE), Integrated Personalized & Precision Oncology Network (IPPON), University of Antwerp, 2Industrial Vision Lab, University of Antwerp, 3Department of Hepatobiliary Transplantation and Endocrine Surgery, University Hospital Antwerp (UZA), 4Department of Oncology, University Hospital Antwerp (UZA), 5Plasma Lab for Applications in Sustainability and Medicine ANTwerp (PLASMANT), University of Antwerp

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Denne protokol beskriver en semi-automatiseret metode til medium- til high-throughput organoid drug screenings og mikroskop-agnostisk, automatiseret billedanalyse software til kvantificering og visualisering af multiparametriske, enkeltorganoide lægemiddelresponser til at fange intratumor heterogenitet.

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Kræftforskning udgave 190 levende cellebilleddannelse organoid lægemiddelscreening multiparametrisk widefield billeddannelse etiketfri analyse tumoroider
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