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Cancer Research

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JoVE Journal Cancer Research
Screening multiparametrico di farmaci organoidi tumorali mediante imaging a cellule vive a campo ampio per analisi di massa e organoidi singoli
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Screening multiparametrico di farmaci organoidi tumorali mediante imaging a cellule vive a campo ampio per analisi di massa e organoidi singoli

Article DOI: 10.3791/64434-v • 12:41 min • December 23rd, 2022
December 23rd, 2022 •
1, 2, 1, 1, 1, 1,3, 2, 1,4, 1,4, 1,5, 1
1Center for Oncological Research (CORE), Integrated Personalized & Precision Oncology Network (IPPON), University of Antwerp, 2Industrial Vision Lab, University of Antwerp, 3Department of Hepatobiliary Transplantation and Endocrine Surgery, University Hospital Antwerp (UZA), 4Department of Oncology, University Hospital Antwerp (UZA), 5Plasma Lab for Applications in Sustainability and Medicine ANTwerp (PLASMANT), University of Antwerp

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Questo protocollo descrive un metodo semi-automatico per screening di farmaci organoidi a medio-alto rendimento e un software di analisi delle immagini automatizzato indipendente dal microscopio per quantificare e visualizzare risposte multiparametriche a singolo organoide per catturare l'eterogeneità intratumorale.

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Ricerca sul cancro numero 190 imaging di cellule vive screening di farmaci organoidi multiparametrico imaging a campo ampio analisi label-free tumoroidi
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