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In vitro Metode for å studere kjønnsbaserte forskjeller i konjunktivale begerceller
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In vitro Metode for å studere kjønnsbaserte forskjeller i konjunktivale begerceller

Article DOI: 10.3791/64456-v • 06:28 min • July 28th, 2023
July 28th, 2023 •
1, 1, 1
1Department of Ophthalmology Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School

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Fenol rødfritt / føtalt bovint serumfritt medium er et bedre alternativ enn avansert RPMI for å eliminere eksogene hormoner uten å endre den normale funksjonen til konjunktivale begerceller i studiet av kjønnsbaserte forskjeller.

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In vitro-metoden studie kjønnsbaserte forskjeller konjunktivale begerceller tørre øyne sykdom okulær overflatehelse prevalens kvinner geldannende mucin sekresjon eksogene kjønnshormoner konsistente resultater fysiologisk funksjon post mortem humane donorer kultur RPMI medium føtalt bovint serum (FBS) fenolrødt BSA cellulær funksjon intracellulær [Ca2+] karbacholstimulering
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