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In vitro Metode til at studere kønsbaserede forskelle i konjunktivale bægerceller
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JoVE Journal Biology
In Vitro Method to Study Sex-Based Differences in Conjunctival Goblet Cells

In vitro Metode til at studere kønsbaserede forskelle i konjunktivale bægerceller

DOI:
10.3791/64456-v

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06:28 min

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July 28, 2023

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, ,
1Department of Ophthalmology Schepens Eye Research Institute, Massachusetts Eye and Ear,Harvard Medical School

Chapters

  • 00:04Introduction
  • 01:40Primary Human Conjunctival Goblet Cell Culture and Passaging
  • 02:52Fura‐2/Acetoxymethyl (AM) Assay for [Ca2+]i Measurement
  • 04:52Results: Measuring [Ca2+]i Concentration in Human Conjunctival Goblet Cells
  • 05:30Conclusion

Summary

Automatic Translation

Automatic Translation

Phenol rødfrit / føtalt bovint serumfrit medium er en bedre mulighed end avanceret RPMI for at eliminere eksogene hormoner uden at ændre den normale funktion af konjunktivale bægerceller i undersøgelsen af kønsbaserede forskelle.

Tags

Keywords: In VitroConjunctival Goblet CellsSex-based DifferencesPrimary CultureIntracellular Calcium MeasurementFura-2 AMOcular Surface DiseasesDrug DevelopmentCell CultureTrypsinization

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