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JoVE Journal Biochemistry
En trippel primær cellekulturmodell av den menneskelige blod-hjernebarrieren for å studere iskemisk hjerneslag in vitro
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En trippel primær cellekulturmodell av den menneskelige blod-hjernebarrieren for å studere iskemisk hjerneslag in vitro

Article DOI: 10.3791/64469-v • 11:06 min • October 6th, 2022
October 6th, 2022 •
1, 1, 1, 1, 1, 1,2
1Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, 2Institute of Physiotherapy and Health Sciences, The Jerzy Kukuczka Academy of Physical Education

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Her beskriver vi metoden for å etablere en trippelcellekulturmodell av blod-hjernebarrieren basert på primære humane hjernemikrovaskulære endotelceller, astrocytter og pericytter. Denne flercellede modellen er egnet for studier av dysfunksjon i nevrovaskulære enheter under iskemisk hjerneslag in vitro eller for screening av legemiddelkandidater.

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