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Studere RNA interaktører av Protein Kinase RNA-aktivert under pattedyr cellen syklus
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
DOI:

10:05 min

March 05, 2019

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Chapters

  • 00:04Title
  • 01:24Preparation of S or M Phase-arrested Cells
  • 02:19Formaldehyde Cross-linking
  • 04:49Immunoprecipitation
  • 07:32Results: Capturing and Analyzing PKR-interacting RNAs During the Cell Cycle Using qRT-PCR
  • 08:45Conclusion

Summary

Automatic Translation

Vi presenterer eksperimentelle metoder for studere RNA-interaktører av double-strandet RNA bindende protein kinase RNA-aktivert (PKR) under pattedyr cellen syklus bruker HeLa celler. Denne metoden bruker formaldehyd krysskobling RNA-PKR komplekser og immunoprecipitation å berike PKR-bundet RNAs. Disse RNAs kan analyseres videre gjennom høy gjennomstrømming sekvensering eller qRT PCR.

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