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Ex vivo Expansion und genetische Manipulation von hämatopoetischen Stammzellen der Maus in Kulturen auf Polyvinylalkoholbasis
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Ex vivo Expansion und genetische Manipulation von hämatopoetischen Stammzellen der Maus in Kulturen auf Polyvinylalkoholbasis

Article DOI: 10.3791/64791-v • 07:55 min • February 10th, 2023
February 10th, 2023 •
*1, *1, 1
1MRC Weatherall Institute of Molecular Medicine, University of Oxford
* These authors contributed equally

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Hier wird ein Protokoll zur Initiierung, Aufrechterhaltung und Analyse hämatopoetischer Stammzellkulturen der Maus unter Verwendung von ex vivo Polyvinylalkohol-basierter Expansion sowie Methoden zur genetischen Manipulation dieser Zellen durch lentivirale Transduktion und Elektroporation vorgestellt.

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Biologie Heft 192
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