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Ex Vivo Uitbreiding en genetische manipulatie van hematopoëtische stamcellen van muizen in op polyvinylalcohol gebaseerde culturen
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Ex Vivo Uitbreiding en genetische manipulatie van hematopoëtische stamcellen van muizen in op polyvinylalcohol gebaseerde culturen

Article DOI: 10.3791/64791-v • 07:55 min • February 10th, 2023
February 10th, 2023 •
*1, *1, 1
1MRC Weatherall Institute of Molecular Medicine, University of Oxford
* These authors contributed equally

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Hier wordt een protocol gepresenteerd voor het initiëren, onderhouden en analyseren van hematopoëtische stamcelculturen van muizen met behulp van ex vivo polyvinylalcoholgebaseerde expansie, evenals methoden om ze genetisch te manipuleren door lentivirale transductie en elektroporatie.

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Biologie Nummer 192
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