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JoVE Journal Bioengineering
Testing av in vitro og in vivo effektivitet av mRNA-lipid nanopartikler formulert ved mikrofluidisk blanding
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Testing av in vitro og in vivo effektivitet av mRNA-lipid nanopartikler formulert ved mikrofluidisk blanding

Article DOI: 10.3791/64810-v • 08:55 min • January 20th, 2023
January 20th, 2023 •
1, 1, 1, 1, 1
1Department of Bioengineering, University of Pennsylvania

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Her presenteres en protokoll for formulering av lipid nanopartikler (LNP) som innkapsler mRNA som koder for firefly luciferase. Disse LNP-ene ble testet for deres styrke in vitro i HepG2-celler og in vivo i C57BL/6-mus.

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Bioteknologi utgave 191 in vitro effektivitet in vivo effektivitet LNP MRNA last nukleaser nedbrytning intracellulær levering ioniserbar lipid fosfolipid kolesterol lipid-PEG konjugat Firefly luciferase transfeksjon effektivitet HepG2 celler bioluminescerende platebasert analyse C57BL / 6 mus intravenøs injeksjon lateral hale vene hele kroppen bioluminescens imaging in vivo bildebehandlingssystem
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