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Developmental Biology

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JoVE Journal Developmental Biology
Differensiering av humane pluripotente stamceller i insulinproduserende øyklynger
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Differensiering av humane pluripotente stamceller i insulinproduserende øyklynger

Article DOI: 10.3791/64840-v • 08:41 min • June 23rd, 2023
June 23rd, 2023 •
*1, *1, 1, 1, 1,2, 3, 1,2,4
1Department of Cellular & Physiological Sciences, Life Science Institute, University of British Columbia, 2School of Biomedical Engineering, University of British Columbia, 3STEMCELL Technologies Inc, 4Department of Surgery, University of British Columbia
* These authors contributed equally

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Differensiering av stamceller til øyceller gir en alternativ løsning til konvensjonell diabetesbehandling og sykdomsmodellering. Vi beskriver en detaljert stamcellekulturprotokoll som kombinerer et kommersielt differensieringssett med en tidligere validert metode for å hjelpe til med å produsere insulinutskillende, stamcelleavledede øyer i en tallerken.

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Utviklingsbiologi utgave 196 insulinproduserende øyklynger betaceller diabetesbehandling protokoll bukspyttkjertelsstamcellesett R-protokoll mikrobrønnplater endokrin differensiering 96-brønns statisk suspensjonsformat in vitro-karakterisering funksjonell evaluering HPSC-avledede øyer stamcellekulturteknikker biologiske analyser
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