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JoVE Journal
Bioengineering

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使用CRISPR SunTag-p65-HSF1激活系统研究米色脂肪生物学和代谢
 
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使用CRISPR SunTag-p65-HSF1激活系统研究米色脂肪生物学和代谢

Article DOI: 10.3791/64849-v 09:52 min January 6th, 2023
January 6th, 2023

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Summary

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Click here for the English version.

该协议提出了在脂肪细胞(AdipoSPH)中使用CRISPR SunTag-p65-HSF1(SPH)作为腺相关病毒(AAV)的替代策略来研究米色脂肪生物学。 体内 注射靶向内源性Prdm16基因的携带AAV的sgRNA足以诱导米色脂肪发育并增强产热基因程序。

Tags

本月在JoVE上,第191期,脂肪组织,产热,新陈代谢,CRISPR
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