Journal
/
Bioengineering
/
使用CRISPR SunTag-p65-HSF1激活系统研究米色脂肪生物学和代谢
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System
Please note that all translations are automatically generated. Click here for the English version.

使用CRISPR SunTag-p65-HSF1激活系统研究米色脂肪生物学和代谢

DOI:
10.3791/64849-v

•

09:52 min

•

January 06, 2023

•

, ,
1Obesity and Comorbidities Research Center,State University of Campinas, 2Department of Structural and Functional Biology, Institute of Biology,State University of Campinas

Chapters

  • 00:05Introduction
  • 00:53Molecular Cloning
  • 04:34In Vivo Injection of Adeno-Associated Virus (AAV) into the Inguinal White Adipose Tissue (iWAT)
  • 06:14In Vitro Differentiation of Stromal Vascular Cells (SVFs) into Beige Adipocytes
  • 07:23In Vitro AAV Infection of SVFs
  • 08:11Results: SPH-Induced Expression of Endogenous Prdm16 for Enhanced Oxygen Consumption
  • 09:23Conclusion

Summary

Automatic Translation

Automatic Translation

该协议提出了在脂肪细胞（AdipoSPH）中使用CRISPR SunTag-p65-HSF1（SPH）作为腺相关病毒（AAV）的替代策略来研究米色脂肪生物学。 体内 注射靶向内源性Prdm16基因的携带AAV的sgRNA足以诱导米色脂肪发育并增强产热基因程序。

Tags

Keywords: CRISPRSunTagP65-HSF1Activation SystemAdipocytesGain-of-functionAdeno-associated VirusPRDM16SgRNACloningVirus Production

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/64849?article_type=v

使用电化学传感器的细菌检测和鉴定
check_url/64849?article_type=v

人类心脏静脉系统使用对比计算机断层扫描灌注固定的标本解剖重建
check_url/64849?article_type=v

在180°弧形动脉试验段的二次流动结构下游的型号IV型支架失败的实验研究
check_url/64849?article_type=v

支架支持胰岛移植糖尿病小鼠附睾脂肪垫
check_url/64849?article_type=v

利用合成生物学工程师活细胞接口，具有可编程材料
check_url/64849?article_type=v

利用 CRISPR-Cas9 系统高效生成和编辑人胰细胞无饲养干细胞
check_url/64849?article_type=v

心脏内侧燃光导管,用于监测细胞代谢,使用渗透哺乳动物心脏的透射吸收光谱
check_url/64849?article_type=v

CRISPR/Cas12a 多路复用基因组编辑糖母细胞和酵母像素艺术的创作
check_url/64849?article_type=v

通过双路西法酶检测系统构建CRISPR质粒和检测哺乳动物细胞的挖空效率
check_url/64849?article_type=v

使用双平面影像学测量 3D 体内肩部运动学

Read Article